Transportation planning is an important factor in terms of urban development. An effective transport system is a major component of social and economic development in urban areas. Public transport accessibility in cities is also an important point in this sense. Public Transport Accessibility Level (PTAL) is a method that measures the accessibility of public transport in a regional and urban area. The purpose of this study is to examine the impact of accessibility of public transport systems on different routes. This impact was investigated by the PTAL method. The accessibility of the tramway line within the province of Karsiyaka district of Izmir City was investigated along with the proposed route. Firstly, the existing route accessibility was calculated. Then, the accessibility of the proposed route was calculated. Both cases are compared.